qPCR,定量PCR,是目前基因表达定量公认的金标准实验方法。

标准规范的qPCR实验包含引物设计合成,引物及实验体系验证,目标产物验证,上机及数据分析等多个步骤。


通常从确定目标基因,设计或查找引物到最终获得理想的实验结果需要花费研究人员几个星期,如果引物验证不成功,需要重新设计验证,将耗费更长时间。


InstantPCR,qPCR,基因表达定量,加入样本cDNA就上机,一步到位。

InstantPCR基因表达定量PCR试剂盒,采用SYBR Green I实时荧光PCR进行基因表达定量。
试剂盒包括验证过的定量PCR引物和PCR MasterMix,加入样本就可以上机实验。

了解更多


严谨的实验验证

Fig.1 PCR扩增曲线

Fig.2 DNA溶解曲线

Fig.3 DNA凝胶电泳

Fig.4 PCR扩增效率统计

案例1:非小细胞肺癌耐药性相关研究
Integrated analysis of DNA methylation and mRNA expression profiling reveals candidate genes associated with cisplatin resistance in non-small cell lung cancer. Epigenetics, 2014. IF: 5.108.

案例2:肝癌相关细胞凋亡研究
Apolipoprotein A1: a novel serum biomarker for predicting the prognosis of hepatocellular carcinoma after curative resection. Oncotarget, 2016. IF: 5.008.

案例3:肺癌AKT信号通路相关研究
Epigenetic downregulation of RUNX3 by DNA methylation induces docetaxel chemoresistance in human lung adenocarcinoma cells by activation of the AKT pathway. The International Journal of Biochemistry & Cell Biology, 2013. IF: 4.24.

案例4:缺氧标志基因相关研究
Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells. J Cell Biochem, 2015. IF: 3.446.

案例5:宫颈癌相关研究
Lu X, Lin Q, Lin M, et al. Multiple-integrations of HPV16 genome and altered transcription of viral oncogenes and cellular genes are associated with the development of cervical cancer. Plos One, 2014. IF: 3.234.

案例6:淋巴瘤相关细胞凋亡研究
Targeting protein neddylation with an NEDD8-activating enzyme inhibitor MLN4924 induced apoptosis or senescence in human lymphoma cells. Cancer Biology & Therapy, 2015. IF: 3.072.

案例7:白血病相关HOX基因研究
Qiu Y, Song B, Zhao G, et al. Expression level of Pre B cell leukemia homeobox 2 correlates with poor prognosis of gastric adenocarcinoma and esophageal squamous cell carcinoma. International Journal of Oncology, 2010. IF: 3.025.

案例8:非小细胞肺癌中miRNA相关研究
EGCG Enhances the Efficacy of Cisplatin by Downregulating hsa-miR-98-5p in NSCLC A549 Cells. Nutrition & Cancer, 2014. IF: 2.47.